Coding

Part:BBa_K1328003:Design

Designed by: Lingfeng Hou   Group: iGEM14_Tsinghua   (2014-10-09)


Insulin (modified protease recognition sites)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PC1 and PC2 protease recognition sites were replaced with that of furin, to allow insulin processing in somatic cells other than natural insulin-secreting cells.


Source

Assembled from 12 short synthesized DNA sequences.

References